Cardiac Regeneration - Methods and Protocols

by Springer US
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Springer US Cardiac Regeneration - Methods and Protocols
Springer US - Cardiac Regeneration - Methods and Protocols

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This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.
The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via

Further information

Illustrations Note:
XIII, 346 p. 83 illus., 69 illus. in color. With online files/update.
Table of Contents:

Part I: Cardiac Injury Models


1. Myocardial Infarction Techniques in Adult Mice

            Elad Bassat, Dahlia Perez, and Eldad Tzahor


2. Apical Resection and Cryoinjury in Neonatal Mouse Heart

            Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien


3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice

            Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt


Myocardial Infarction in Pigs

            Andrea Bähr, Nadja Hornaschewitz, and Christian Kupatt


5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish

            Ines J. Marques, Andrés Sanz-Morejón, and Nadia Mercader


6. Cardiac Resection Injury in Zebrafish

            Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi


7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish

            Fei Sun, Adam R. Shoffner, and Kenneth D. Poss


8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration

            Yijen L. Wu


Part II: Ex Vivo and In Vivo Approaches


9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes

            Marina Leone and Felix B. Engel


10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes

            Lu Han, Jocelyn Mich-Basso, and Bernhard Kühn


11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells

            Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra


12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells

            Jun Xu, Li Wang, Jiandong Liu, and Li Qian


13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs

            Abbigail Helfer and Nenad Bursac


14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells

            Ling Gao and Jianyi Zhang


15. Isolation[SL1] [DC2]  and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts

            Honghai Liu, Kevin Bersell, and Bernhard Kühn


16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish

            Sierra Duca and Jingli Cao


17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters

            Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, and Sean M. Wu


Part III: Visualizing and Manipulating Heart Regeneration


18. In Vivo Clonal Analysis of Cardiomyocytes

            Ngoc B. Nguyen, G. Esteban Fernandez, Yichen Ding, Tzung Hsiai, and Reza Ardehali


19. High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry

            Frank Gyngard, Louise Trakimas, and Matthew L. Steinhauser


20. AAV Gene Transfer to the Heart

            Suya Wang, Yuxuan Guo, and William T. Pu


21. In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy

            Nishat Sultana, Mohammad Tofael Kabir Sharkar, Yoav Hadas, Elena Chepurko, and Lior Zangi


22. Generation and Manipulation of Exosomes

            Shiqi Hu, Li Qiao, and Ke Cheng


23. Epigenetic Assays in Purified Cardiomyocyte Nuclei

            Matthew C. Hill and James F. Martin


24. Genetic Lineage Tracing of Non-Cardiomyocytes in Mice

            Zhongming Chen and Jop H. van Berlo


25. Experimental Hypoxia as a Model for Cardiac Regeneration in Mice

            Yuji Nakada and Hesham A. Sadek



1st ed. 2021
Number of Pages:
Poss, Kenneth D.;Poss
Kühn, Bernhard;Kühn
Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts

Media Type:
Springer US

Master Data

Product Type:
Paperback book
Release date:
29 August 2020
Package Dimensions:
0.259 x 0.196 x 0.023 m;
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