Cardiac Regeneration - Methods and Protocols

by Springer US
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Springer US Cardiac Regeneration - Methods and Protocols
Springer US - Cardiac Regeneration - Methods and Protocols

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Description

This detailed book addresses major goals of regenerative medicine and the cardiovascular research community with techniques to replenish lost cardiomyocytes, avoid scar-associated pathology, and improve myocardial infarction (MI) outcomes. The collection begins with a section on cardiac injury models, including zebrafish, neonatal and adult mice, and pigs, and continues with sections covering culturing cardiomyocytes from different species as well as methods for labeling or manipulation of cardiac tissue for the purpose of answering questions in regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Cardiac Regeneration: Methods and Protocols provides the latest models and methods used in the field of heart regeneration, designed for researchers interested in establishing these assays in their laboratories to reproduce or extend findings, and for familiarizing themselves with the field if it is new to them.
The chapter “Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Further information

Illustrations Note:
XIII, 346 p. 83 illus., 69 illus. in color. With online files/update.
Language:
English
Table of Contents:


Part I: Cardiac Injury Models




 




1. Myocardial Infarction Techniques in Adult Mice



            Elad Bassat, Dahlia Perez, and Eldad Tzahor



 




2. Apical Resection and Cryoinjury in Neonatal Mouse Heart



            Hua Shen, Ali Darehzereshki, Henry M. Sucov, and Ching-Ling Lien



 




3. Left-Ventricular Pressure Volume Loop Measurements Using Conductance Catheters to Assess Myocardial Function in Mice



            Tilman Ziegler, Karl-Ludwig Laugwitz, and Christian Kupatt



 




4.
Myocardial Infarction in Pigs



            Andrea Bähr, Nadja Hornaschewitz, and Christian Kupatt



 




5. Ventricular Cryoinjury as a Model to Study Heart Regeneration in Zebrafish



            Ines J. Marques, Andrés Sanz-Morejón, and Nadia Mercader



 




6. Cardiac Resection Injury in Zebrafish



            Delicia Z. Sheng, Dawei Zheng, and Kazu Kikuchi



 




7. A Genetic Cardiomyocyte Ablation Model for the Study of Heart Regeneration in Zebrafish



            Fei Sun, Adam R. Shoffner, and Kenneth D. Poss



 




8. Cardiac MRI Assessment of Mouse Myocardial Infarction and Regeneration



            Yijen L. Wu



 




Part II: Ex Vivo and In Vivo Approaches



 




9. Isolation, Culture, and Live Cell Imaging of Primary Rat Cardiomyocytes



            Marina Leone and Felix B. Engel



 




10. Generation of Human Induced Pluripotent Stem Cells and Differentiation into Cardiomyocytes



            Lu Han, Jocelyn Mich-Basso, and Bernhard Kühn



 




11. Differentiation of Human Induced Pluripotent Stem Cells into Epicardial-Like Cells



            Navid A. Nafissi, Paige DeBenedittis, Michael C. Thomas, and Ravi Karra



 




12. In Vitro Conversion of Murine Fibroblasts into Cardiomyocyte-Like Cells



            Jun Xu, Li Wang, Jiandong Liu, and Li Qian



 




13. Frame-Hydrogel Methodology for Engineering Highly Functional Cardiac Tissue Constructs



            Abbigail Helfer and Nenad Bursac



 




14. Efficient Protocols for Fabricating a Large Human Cardiac Muscle Patch from Human Induced Pluripotent Stem Cells



            Ling Gao and Jianyi Zhang



 




15. Isolation[SL1] [DC2]  and Characterization of Intact Cardiomyocytes from Frozen and Fresh Human Myocardium and Mouse Hearts



            Honghai Liu, Kevin Bersell, and Bernhard Kühn



 




16. Ex Vivo Techniques to Study Heart Regeneration in Zebrafish



            Sierra Duca and Jingli Cao



 




17. Purification of Pluripotent Stem Cell-Derived Cardiomyocytes Using CRISPR/Cas9-Mediated Integration of Fluorescent Reporters



            Francisco X. Galdos, Adrija K. Darsha, Sharon L. Paige, and Sean M. Wu



 




Part III: Visualizing and Manipulating Heart Regeneration




 




18. In Vivo Clonal Analysis of Cardiomyocytes



            Ngoc B. Nguyen, G. Esteban Fernandez, Yichen Ding, Tzung Hsiai, and Reza Ardehali



 




19. High Fidelity Quantification of Cell Cycle Activity with Multi-Isotope Imaging Mass Spectrometry



            Frank Gyngard, Louise Trakimas, and Matthew L. Steinhauser



 




20. AAV Gene Transfer to the Heart



            Suya Wang, Yuxuan Guo, and William T. Pu



 




21. In Vitro Synthesis of Modified RNA for Cardiac Gene Therapy



            Nishat Sultana, Mohammad Tofael Kabir Sharkar, Yoav Hadas, Elena Chepurko, and Lior Zangi



 




22. Generation and Manipulation of Exosomes



            Shiqi Hu, Li Qiao, and Ke Cheng



 




23. Epigenetic Assays in Purified Cardiomyocyte Nuclei



            Matthew C. Hill and James F. Martin



 




24. Genetic Lineage Tracing of Non-Cardiomyocytes in Mice



            Zhongming Chen and Jop H. van Berlo



 




25. Experimental Hypoxia as a Model for Cardiac Regeneration in Mice



            Yuji Nakada and Hesham A. Sadek



 [SL1]



 [DC2]

Edition:
1st ed. 2021
Number of Pages:
346
Editor:
Poss, Kenneth D.;Poss
Kühn, Bernhard;Kühn
Remarks:
Includes cutting-edge techniques


Provides step-by-step detail essential for reproducible results


Contains key implementation advice from the experts

Media Type:
Hardcover
Publisher:
Springer US

Master Data

Product Type:
Paperback book
Release date:
29 August 2020
Package Dimensions:
0.259 x 0.196 x 0.023 m;
GTIN:
09781071606674
DUIN:
F2RJF69HHAO
£118.28
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