IMPEDANCE SPECTROSCOPY FOR CELLULAR ANALYSIS - Novel electrode materials and device designs for single cell analysis

by Malleo, Daniele
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Malleo, Daniele IMPEDANCE SPECTROSCOPY FOR CELLULAR ANALYSIS - Novel electrode materials and device designs for single cell analysis
Malleo, Daniele - IMPEDANCE SPECTROSCOPY FOR CELLULAR ANALYSIS - Novel electrode materials and device designs for single cell analysis

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Description

The main focus of this work is the novel use of impedance spectroscopy as a tool for single cell analysis. Impedance spectroscopy is a non-invasive, label-free, analytical technique that has found widespread use in disparate scientific and technological fields, but, until now, has been a somewhat neglected tool for the analysis of single cells, mainly due to the technological difficulties intrinsic to the fabrication of microelectrodes and the bio-compatible microenvironments within which the cells under study need to be trapped and isolated. The work begins with a comprehensive literature review and an introductory chapter to the theoretical foundations of impedance spectroscopy. A novel device that can perform continuous differential impedance spectroscopic analysis of single cells hydrodynamically captured in an array inside a microfluidic channel is presented. Iridium oxide and polyelectrolyte coatings are studied as novel electrode materials for impedance spectroscopy, and their advantages are explored in comparison with metals commonly used in microfabricated devices, such as gold or platinum.

Contributors

Author:
Malleo, Daniele

Further information

Biography Artist:
Daniele Malleo is an engineer developing microdevices for single cell gene expression analysis. He obtained a PhD degree in Electronic Engineering (2009) and an MSc degree in Microelectronic Engineering (2004) from the University of Southampton (UK). From 2006 to 2008 he was a visiting scholar in the Bioengineering department at UC Berkeley.
Language:
Englisch
Number of Pages:
220
Media Type:
Taschenbuch
Publisher:
LAP Lambert Academic Publishing

Master Data

Product Type:
Paperback book
Release date:
19 February 2010
Package Dimensions:
0.22 x 0.15 x 0.013 m; 0.376 kg
GTIN:
09783838340838
DUIN:
M4LA00CM214
£68.99
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