Functional Genomic Analysis of Pseudorabies Virus - Functional Analysis of Pseudorabies Virus Genes by Real-Time RT PCR and Knock-Out Technique

by Tombacz, Dora
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Tombacz, Dora Functional Genomic Analysis of Pseudorabies Virus - Functional Analysis of Pseudorabies Virus Genes by Real-Time RT PCR and Knock-Out Technique
Tombacz, Dora - Functional Genomic Analysis of Pseudorabies Virus - Functional Analysis of Pseudorabies Virus Genes by Real-Time RT PCR and Knock-Out Technique

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Description

The pseudorabies virus (PRV) is a neurotropic herpesvirus, it is a pathogen of swine, causing Aujeszky's disease. PRV is a widely used model organism for studies in pathogenesis and molecular biology. This virus is also utilized as a neural circuit tracer. We have developed (1) a real-time RT-PCR method for the global analysis of PRV gene expression; (2) a novel calculation method to examine the relative expression ratios, and which is applicable for the analysis of gene expression in any genetic system that progressively changes in time; (3) PRV-based troponeon expressing transsynaptic tracers which label synaptically connected nerve cells; (4) transgenic viruses encoding differentially colored fluorescent proteins (FPs) and multiple colored FPs to differentiate the brain regions; (5) a method for a short-term culture of isolated cardiac muscle cells that retain their morphological and physiological integrity; (6) a PRV-based system for delivery of foreign genes to cardiomyocytes.

Contributors

Author:
Tombacz, Dora
Boldogkoi, Zsolt

Further information

Language:
Englisch
Number of Pages:
76
Media Type:
Taschenbuch
Publisher:
LAP Lambert Academic Publishing

Master Data

Product Type:
Paperback book
Release date:
03 January 2011
Package Dimensions:
0.22 x 0.15 x 0.005 m; 0.159 kg
GTIN:
09783843389488
DUIN:
QAU0CH9PGT3
£41.99
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