Indirect Regeneration of Japonica Rice through Anther Culture - Effect of Growth Regulator Concentrations on Callus Induction and Regeneration in Japonica Rice Varieties
by Sharma, Avinash
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Rice (Oryza sativa L.) is one of the most important food crops of Southeast Asia, which feeds half of the world population. Anther culture is an efficient and convenient technique for rapid production of doubled haploids which are useful in crop breeding programs. The combination of different growth regulators facilitates callus induction on N6 medium and regeneration on MS medium. Highest callus induction frequency was recorded in growth regulator concentrations of 2, 4-D 2.0 mg/l + Kinetin 1.0 mg/l (34.54%) followed by in growth regulator concentrations 2, 4-D 1.0 mg/l + NAA 2.0 mg/l + Kinetin 0.5 mg/l (33.38%). Highest regeneration frequency was recorded in growth regulator concentrations of Kinetin 0.5 mg/l + BAP 2.0 mg/l + NAA 1.0 mg/l (69.44%) followed by in growth regulator concentrations of Kinetin 2.0 mg/l + BAP 1.0 mg/l + NAA 1.0 mg/l (40.00%). This is clearly indicated that type of growth regulator along with its concentration plays important role in callus induction and regeneration in japonica rice varieties.
LAP Lambert Academic Publishing
I am Avinash Sharma, belonging to Nalanda District, Bihar, India. I studied B.Sc.(Agri.) at Central Agricultural University, Imphal in 2013 and pursued M.Sc. (Agri.) with specialization in Agricultural Biotechnology from University of Agricultural Sciences, Bangalore in 2015. I achieved ICAR-NET 2015 and published five (5) research papers.
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12 February 2018
0.22 x 0.15 x 0.004 m; 0.145 kg